Cisplatin assay

WebMar 1, 2024 · Cisplatin (cis-diamminedicholroplatinum II, Fig. 1) is a platinum complex bound to two chloride atoms and two ammonia molecules which makes it highly soluble … WebMar 15, 2024 · Here, MGC-803 and SGC-7901 gastric cancer cells were treated with different concentrations of cisplatin. Comet assay was used to detect DNA damage and the results confirmed the dose-effect of cisplatin-induced DNA damage in gastric cancer cells. URI knockdown cell lines were established with siRNA transfection. Cell viability and …

A Simple And Sensitive HPLC Method For Determination …

WebMar 1, 2024 · The developed and validated assay presented here is a highly sensitive LC-MS/MS method developed and validated for the quantitative determination of cisplatin following derivatization with diethyldithiocarbamate (DDTC) in order to detect platinum (Pt) of cisplatin, suitable for pharmacokinetic studies in rats and to further use it to study … WebApr 11, 2024 · Cisplatin is a widely used chemotherapeutic agent for treatment of various cancers. However, treatment with cisplatin is associated with drug resistance and … simply rc pro https://kwasienterpriseinc.com

Quantitative analysis of cisplatin sensitivity of human ... - PubMed

WebNov 1, 2008 · Cisplatin is an anti-tumor agent widely employed in cancer chemotherapy. A specific and selective method for the quantitative determination of cisplatin in human plasma and its applications to ... After observing the enhanced cell-killing efficacy of the combination of CDDP and BV10 in various cancer cell lines and studying cell death processes, we further investigated the anti-cancer effect of this combination in the xenograft mouse models of lung, ovarian and cervical cancers. The typical dose of … See more The results of the effect of the combination of CDDP with BV10 on in vitro cell viability and clonogenic death are shown in Fig. 3. Figure 3A shows the … See more To demonstrate the DET mechanism of the combination, we made real-time measurements of the reaction between CDDP and BV10 … See more Figure 4A shows representative images of HeLa cells treated by CDDP with/without the combination with BV10 for 12 h, where apoptotic cells were … See more WebOct 25, 2016 · The MTT assay is currently the most extensively used method for IC50 measurements. Here, we dissected the critical reasons behind MTT-dependent IC50 inconsistencies. We showed that IC50 errors caused by the technical deficiencies of the MTT assay are large and not adjustable (range: 300-11,000%). ray\u0027s cracked head repair

Cisplatin (Cisplatin Injection): Uses, Dosage, Side Effects ... - RxList

Category:USP Monographs: Cisplatin - Pharmacopeia.cn

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Cisplatin assay

Cumulative DNA damage by repeated low-dose cisplatin injection …

WebMay 26, 2024 · Cisplatin is a well-known chemotherapeutic drug for solid tumors and leukemia with muscle toxicity, and long-term cisplatin medication can induce aberrant ROS accumulation in muscle cells and result in systemic muscle impairments ( Sakai et al., 2014; Conte et al., 2024 ). WebJan 18, 2024 · Cisplatin was administered by intraperitoneal injection in mouse. DNA damage, excision repair, and gene expression were assayed by Damage-seq, XR-seq, and RNA-seq. b PCA reveals tissue-specific...

Cisplatin assay

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WebApr 2, 2024 · The assay was optimized using Analysis of Variance (ANOVA) to identify potential experimental confounders and Z-factor (Z), … WebElectron microscopywas used to observe ultrastructure changes in cell. Results: The depletion of reduced glutathione caused by cisplatin and the inactivation of glutathione peroxidase played the vital role in the underlying mechanism.

WebIntroduction: Cisplatin has been indicated for several malignancies all over the world for many years. Increasing patient tolerance for high dose of chemotherapeutics and … WebSep 23, 2016 · The subsequent intracellular signaling pathway analysis indicated that pAkt and p62 expression levels correlated with alterations in the IC50 values for cisplatin in ovarian cancer, providing an...

WebAug 13, 2024 · Annexin V/propidium iodide (PI) apoptosis assay Cells were seeded onto 6-well plates at a density of 2.5 × 10 5 cells/well and assigned into four groups. In the single treatment group, cells were... WebAbstract Cisplatin (DDP) is a common therapeutic option for non-small cell lung carcinoma (NSCLC). However, some patients fail to respond to the DDP chemotherapy. ... Cells were lysed with radioimmunoprecipitation assay (RIPA) buffer and incubated with anti-USP14 (ab70311; Abcam), anti-EGFR (ab52894; Abcam), or normal IgG (sc-2027; Santa Cruz ...

WebCisplatin is also sometimes used to treat head and neck cancer (including cancer of the mouth, lip, cheek, tongue, palate, throat, tonsils, and sinuses), lung cancer, cancer of the … ray\\u0027s crab shack fremont caWebAug 8, 2010 · Cisplatin is one of the most effective anticancer agents widely used in the treatment of solid tumors. It is generally considered as a cytotoxic drug which kills cancer cells by damaging DNA and inhibiting DNA synthesis. How cells respond to cisplatin-induced DNA damage plays a critical role in deci … ray\u0027s cousin gerardWebAug 6, 2015 · The chemotherapeutic use of cisplatin is limited by its severe side effects. In this study, by conducting different omics data analyses, we demonstrated that cisplatin … ray\u0027s country hamWebUsing transporter-mediated cellular uptake assay, we identified wedelolactone (WEL), a medicinal plant-derived natural compound, is a competitive inhibitor of OCT2 and a noncompetitive inhibitor of MATE1. Wedelolactone showed a selectivity to inhibit OCT2 rather than MATE1. ray\\u0027s country smokehouse gastoniaWebCisplatin An anticancer agent WARNING: This product can expose you to chemicals including Cisplatin, which is [are] known to the State of California to cause cancer. For … ray\\u0027s cracked head seymour indianaWebAbstract Cisplatin (DDP) is a common therapeutic option for non-small cell lung carcinoma (NSCLC). However, some patients fail to respond to the DDP chemotherapy. ... Cells … ray\\u0027s cousin gerardhttp://www.pharmacopeia.cn/v29240/usp29nf24s0_m17910.html ray\u0027s crab shack fremont